Services

In vitro diagnostic lab tests

At Epigenovix Diagnostics we are dedicated to delivering the most accurate results of early-stage cancer screening from liquid biopsies and solid tissues. According to the National Cancer Institutes and the American Cancer Society the 5-year survival rate when cancer is detected at stage I is 89 percent versus only 10 percent when the disease is detected at stage IV. Therefore, we are committed to providing solution for early cancer detection.

Epigenetic DNA Methylation Analysis

de-novo snp analysis
Detecting cancer, Diabetes, Neurodegenerative Disease, Aging, Environmental Stressors and Age-related Diseases and other chronic diseases. Genomic variant Single Nucleotide Polymorphism (SNP) analysis- Detecting diseases, drug response and other phenotypes.

De-novo variant detection in target SNP region

The dna methylation analysis

DNA methylation is an important epigenetic modification that regulates transcription and thus affects gene expression. It is a popular research object in the fields of embryonic development, postnatal development, cancer, and the influence of environmental hormones on epigenetic inheritance. In mammals, DNA methylation is found to occur predominantly on cytosines followed by guanine residues (CpG). This type of methylation is referred to as CpG methylation, and cytosine methylated at the fifth carbon of the pyrimidine ring is called 5-methylcytosine (5mC). Due to the influences of environment, disease, age and gender factors, DNA methylation is in a dynamic change process, in which DNA methylation can be established and maintained by DNA methyltransferase (DNMT) and it can be demethylated by DNA demethylase.
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Aberrant change of DNA methylation content and patterns is a significant factor in the occurrence and development of diseases. In cancer research, abnormal change in DNA methylation considered to be one of biomarkers for early diagnosis and the promising target for developing therapeutic approaches.

De-novo variant detection in target SNP region

The dna methylation analysis

DNA methylation is an important epigenetic modification that regulates transcription and thus affects gene expression. It is a popular research object in the fields of embryonic development, postnatal development, cancer, and the influence of environmental hormones on epigenetic inheritance. In mammals, DNA methylation is found to occur predominantly on cytosines followed by guanine residues (CpG). This type of methylation is referred to as CpG methylation, and cytosine methylated at the fifth carbon of the pyrimidine ring is called 5-methylcytosine (5mC). Due to the influences of environment, disease, age and gender factors, DNA methylation is in a dynamic change process, in which DNA methylation can be established and maintained by DNA methyltransferase (DNMT) and it can be demethylated by DNA demethylase.
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Aberrant change of DNA methylation content and patterns is a significant factor in the occurrence and development of diseases. In cancer research, abnormal change in DNA methylation considered to be one of biomarkers for early diagnosis and the promising target for developing therapeutic approaches.

Single nucleotide polymorphisms

(SNPs) are a type of mutation that occurs at a high frequency in a population or racial/ethnic groups (polymorphism) involving variation of a single base pair. Single nucleotide polymorphisms, or SNPs in the human genome correlate with disease, drug response, and other phenotypes. Epigenovix Diagnostic approach for DNA methylation analysis is based on bisulfite conversion followed by sequencing (high-throughput quantitative pyrosequencing approach).

Bisulfite Conversion Service

Bisulfite conversion assay is based on the chemical conversion of unmethylated cytosine to uracil, while methylated cytosine is exempt from this conversion, after which various analyses can be performed on the altered sequences to retrieve this information.

Bisulfite Sequencing Service

The combination of bisulfite conversion and high-throughput sequencing (pyrosequencing service) offers the most quantitative method for DNA methylation analysis at gene/promoter specific loci and Single Nucleotide Polymorphism (SNP) at single-base resolution. 

Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR)

Analysis: Detecting differential gene expression in viral infection, cancer, and a host of chronic diseases.

qRT-PCR monitors the amplification of targeted DNA and reversed transcribed RNA molecules during the PCR reaction. It monitors the amplification of a targeted DNA molecule during the PCR, not at its end, as in conventional PCR. This method can be quantitative or semi-quantitative detection of nucleic acid from viruses (such as COVID19) and bacteria infection in specimens. The assay is composed of two principal steps: (1) extraction of total nucleic acid (DNA and RNA) from patient specimens, (2) one-step reverse transcription and PCR amplification with gene-specific transcripts with specific primers and probes.

Specimen used in lab testing:

  • Urine
  • Blood
  • Saliva
  • Stool
  • Human tissues

Specimen preparation:

We have extensive expertise in delivery high quality sample preparation from various biological specimen including circulating tumor DNA (ctDNA), RNA samples and proteins from biological specimen for our customers. Nucleic Acids (DNAs and RNAs) are extracted from specimen (human and non-human). For DNA methylation analysis and qRT-PCR analysis gene specific probes and probe library generation (Bioinformatics and laboratory). Other assays are designed such as using customer-provided SNP sequence information.

Genetic manipulation:

We are highly skilled in delivering quality biological sample preparations from human biospecimen and provide additional services including recombinant vector cloning (gene synthesis/clone services), gene promoter vector construction, luciferase reporter assay construction, and downstream applications.

Epigenovix Educational Services:

Practical Steps to Healthy Living

Our educational approach is to inform the general public topic related to cancer prevention and early detection, health disparities and risk factors associated with chronic diseases. You will gain new insights in this fun and yet challenging virtual educational teaching in practical education methodology to help individuals and community in avoiding adverse health risk factors and to adapt healthier lifestyle choices to avoid and eliminate chronic diseases.

Cancer Prevention and Early Disease Detection

This educational workshop teaches about the common risk factors associated with particular types of cancer, including genetic predisposition, radiation and chemical carcinogens, diet, hormonal factors, infection, and smoking. We present the scientific evidence supporting the positive role of healthy nutrition, exercise, and diet in lowering cancer risk, as well as the dangers posed by a dysfunctional immune system compromised by chronic infection, unhealthy lifestyles, stress, and poor psychological health. Finally, we provide an unbiased assessment of a number of controversies surrounding cancer causes and prevention, including screening and genetic testing, vitamin supplementation, genetically modified foods, chemical food additives, and cellular phones and deodorants as potential cancer-causing agents.

Included in the educational session:

  • Risk Factors for Commonly Diagnosed Cancer- including Bladder Cancer, Brain Cancer, Breast Cancer, Cervical Cancer, Head & Neck Cancer, Liver Cancer, Lung Cancer, Pancreatic Cancer, Prostate Cancer and Skin Cancer -Behaviors and Controversies
  • Nutrition and Cancer
  • Body weight and Cancer
  • Immune System and Cancer
  • Controversies Concerning Cancer Screening; Benefits and Disadvantages
  • Controversies Surrounding Dietary Supplements and Foods for Cancer Prevention
  • Controversies Regarding Commonly Perceived Risk Factors and Cancer

Health Disparities Education

We use multiple lenses to discuss the complex causes of health disparities affecting minorities, in particular African Americans, and explains how this knowledge can be used to reduce their destructive effects. We pinpoint genetic, non-genetic, and epigenetic factors underlying health conditions common to the population—including heart disease, hypertension, diabetes, and cancer—the author traces intricate links among these factors in the current environmental and social context. We teach a section on non-genetic factors in health disparities, such as social determinants and health behaviors, adds depth to the ongoing discourse on public health and health policy objectives. And the chapters on gene/environment interactions outline the vast potential for developing new multidisciplinary frontiers in shrinking health inequities and personalizing care.

Included in the educational session:

  • The African diaspora and disease-specific disparities
  • The genetic basis to health disparities
  • The role of epigenetics
  • Economic factors and health
  • Psychological issues and how they affect disparities
  • Gene-environment interactions in health disparities
  • Race, a biological or social concept

Risk Factors Driving Chronic Diseases

We discuss the factors driving global obesity epidemic and this is associated with thousands of chronic diseases. At the root cause of excess body weight or obesity is diet high in caloric content, ultra-processed foods padded with sugars, salt, and fat as well as sedentary lifestyle. Our goal then is to consume healthy nutritious foods and avoid excess caloric intake in the first place. Yet, many dietary approaches as well as exercise regime only provide short-term solutions and conventional treatment of chronic diseases at best can only manage these conditions. The challenge with modern dietary intervention and physical activity clearly indicates that the current obesity epidemic defies simple solutions. This workshop discusses how the human body has designed complex and integrated metabolic process that involves several factors such as hormones, gut microbiome, circadian rhythm/sleep pattern, genetic traits, and exposures to the current obesogenic environment in regulating energy intake from diet and energy expenditure as well as energy storage in the form of fat. Ultimately we want to gain insights from our ancestors who did not have chronic diseases and presents the scientific evidence in support of the role of poor dietary components and sedentary lifestyle and their association with chronic diseases whereas healthy dietary interventions can reverse and even eliminate chronic diseases.

Included in the educational session:

  • Dietary Components
  • Food Metabolic Pathways
  • The Exercise Paradox
  • Gut Microbiome, the Other Brain
  • Genetic Variants and Diet
  • Diet and Diseases
  • The Battle of Excess Body Fat
  • Eliminating Chronic Diseases through Lifestyle Changes

Cancer Prevention and Early Disease Detection

This educational workshop teaches about the common risk factors associated with particular types of cancer, including genetic predisposition, radiation and chemical carcinogens, diet, hormonal factors, infection, and smoking. We present the scientific evidence supporting the positive role of healthy nutrition, exercise, and diet in lowering cancer risk, as well as the dangers posed by a dysfunctional immune system compromised by chronic infection, unhealthy lifestyles, stress, and poor psychological health. Finally, we provide an unbiased assessment of a number of controversies surrounding cancer causes and prevention, including screening and genetic testing, vitamin supplementation, genetically modified foods, chemical food additives, and cellular phones and deodorants as potential cancer-causing agents.

Included in the educational session:

  • Risk Factors for Commonly Diagnosed Cancer- including Bladder Cancer, Brain Cancer, Breast Cancer, Cervical Cancer, Head & Neck Cancer, Liver Cancer, Lung Cancer, Pancreatic Cancer, Prostate Cancer and Skin Cancer -Behaviors and Controversies
  • Nutrition and Cancer
  • Body weight and Cancer
  • Immune System and Cancer
  • Controversies Concerning Cancer Screening; Benefits and Disadvantages
  • Controversies Surrounding Dietary Supplements and Foods for Cancer Prevention
  • Controversies Regarding Commonly Perceived Risk Factors and Cancer

Health Disparities Education

We use multiple lenses to discuss the complex causes of health disparities affecting minorities, in particular African Americans, and explains how this knowledge can be used to reduce their destructive effects. We pinpoint genetic, non-genetic, and epigenetic factors underlying health conditions common to the population—including heart disease, hypertension, diabetes, and cancer—the author traces intricate links among these factors in the current environmental and social context. We teach a section on non-genetic factors in health disparities, such as social determinants and health behaviors, adds depth to the ongoing discourse on public health and health policy objectives. And the chapters on gene/environment interactions outline the vast potential for developing new multidisciplinary frontiers in shrinking health inequities and personalizing care.

Included in the educational session:

  • The African diaspora and disease-specific disparities
  • The genetic basis to health disparities
  • The role of epigenetics
  • Economic factors and health
  • Psychological issues and how they affect disparities
  • Gene-environment interactions in health disparities
  • Race, a biological or social concept

Risk Factors Driving Chronic Diseases

We discuss the factors driving global obesity epidemic and this is associated with thousands of chronic diseases. At the root cause of excess body weight or obesity is diet high in caloric content, ultra-processed foods padded with sugars, salt, and fat as well as sedentary lifestyle. Our goal then is to consume healthy nutritious foods and avoid excess caloric intake in the first place. Yet, many dietary approaches as well as exercise regime only provide short-term solutions and conventional treatment of chronic diseases at best can only manage these conditions. The challenge with modern dietary intervention and physical activity clearly indicates that the current obesity epidemic defies simple solutions. This workshop discusses how the human body has designed complex and integrated metabolic process that involves several factors such as hormones, gut microbiome, circadian rhythm/sleep pattern, genetic traits, and exposures to the current obesogenic environment in regulating energy intake from diet and energy expenditure as well as energy storage in the form of fat. Ultimately we want to gain insights from our ancestors who did not have chronic diseases and presents the scientific evidence in support of the role of poor dietary components and sedentary lifestyle and their association with chronic diseases whereas healthy dietary interventions can reverse and even eliminate chronic diseases.

Included in the educational session:

  • Dietary Components
  • Food Metabolic Pathways
  • The Exercise Paradox
  • Gut Microbiome, the Other Brain
  • Genetic Variants and Diet
  • Diet and Diseases
  • The Battle of Excess Body Fat
  • Eliminating Chronic Diseases through Lifestyle Changes
The goal of Epigenovix Diagnostics is to develop sensitive and specific biomarkers for disease detection.
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Contact Information

  • Phone: +01-281 507 2409
  • email: bernardka@epigenovix.com
  • Address: 2479 FIVE FATHOM CIRCLE WOODBRIDGE VA 22191
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